Manfaat Pemberian Madu terhadap Perubahan Kadar Malondialdehyde Plasma, Disease Activity Index serta Makroskopik Kolon dan Histopatologi Epitel Mukosa Kolon Mencit (Mus Musculus L.) Jantan yang Diberi Rhodamin B

Abstract

Rhodamin B is a synthetic dye that is not designated as food additivies, dangerous if it enters the body, cause oxidative stress characterized by elevated levels of malondialdehyde (MDA), if continued lead to system damage cell membranes and cell death. The purpose of this study was to determine the benefits of honey to changes in plasma malondialdehyde (MDA) levels, disease activity index, macroscopic of colon and hystophatology of epithelium mucosa/ colonic of mice fed RhodaminB. This study was the laboratoric experimental research by the post test only control group design. The subjects were male mice (Mus musculus L.) Strains DD Webster, 7-10 weeks, 25-40 grams Obtained from the Faculty of mathematics and natural science USU Subjects were divided into 4 groups: control group (PO), Rhodamin B group 2% (P 1), Honey 0. 08ml/20 grams mice/day group (P2), and Rhodamin B 2% and honey 0.08ml/20 gram mice/day group (P3). 56 tail divided by 4 treatment groups: (1) PO (control) were given 0.3 ml aquades, (2) Rhodamin B dose 0.2% I hr, 0.3 ml, (3) doses of 0.08 ml/20 honey gr BB I hr, 0.3 ml, (4) Rhodamin B dose of 0. 2% I hr, 0. 3 ml of honey + 0, 08ml/20 gr BB I hr, 0. 3 ml, treated for 21 days, then continued for 7 days without being treated to see the restoration of his cell. Examination of plasma MDA levels with a spectrophotometer, damages the colonic mucosa with a magnifying glass and light microscopy. Ethical clearance obtained from the Animal Research Ethics commission FMIP A USU The results show when treated and when cell recovery (recovery) found a statistically significant increase in plasma MDA levels, damage to the colonic mucosa in the macroscopic and histopathology in the group given Rhodamin B (P 1) and the group given Rhodamin Band honey (P3) to the control group (PO) and the provision of honey (P2) (p <0.05). While the assessment of Disease Activity Index showed no change in clinical disease as a result of inflammation of the colon Rhodamin B (Value= 0) in all treatment groups. This study found that the bioactive content of honey can not protect the damage caused by Rhodamin B