Manfaat Pemberian Madu terhadap Perubahan Kadar ALT, AST serta Perubahan Makroskopik dan Histopatologi Hati Mencit Jantan (Mus musculus L.) yang Diberi Rhodamin B

Abstract

Rhodamin B could destroyed hepatocyte cells which could increased blood ALT and AST, which could indicated the liver cells destruction. The purpose of this research is to study the benefit of honey improving ALT and AST on hepatocite cells, macroscopicly and histopathological of the liyer male mice (Mus musculus L.) which administated of Rhodamin B and to stu$t the ability of hepatosites to bnprove themselves after rhodamin

B and honey terminated- This study was the laboratoric experimental research by complete random design.

The subjects were male mice Mus musculus L Strain DD Webster of 8-tl week, with 25- 40 grams. Obtained from the Faculty of mathemotics and natural science USU. Subjects were divided into 4 groups: control group (P0), Rhodamin B group 2% (Pl), Honey 0.08ng/20 grams mice/day group (P2), and Rhodamin B 2?6 and honey 0.08mg/20 gram mice/day group (P3). Then performed tests of normality and homogeneily of data. when the data distributed normally and homogeneous then performed ANOVA test, if (p <0.05) followed by a test post hock analysis Benferroni lewl of 5o%, if not normally distributed data and is not homogeneous the Mann Whitney test. This results had obtained is performed increasing of ALT and AST were signiJicantly different from group Pl with P0, P2, PlR, P2R b<0.05) and did not significantly dffirent with the group P3, PL(R), P3(R) (p>0.05). Degrees of hepatic necrosis and congestion on group Pl was significantly increased compared with P0, P2, PO(R) and P2(R) (p<0.05) and did not signilicantly dffirent with the Pi, PI(R), P3(R) (Ir>0.05). The macroscopic of the liver showed signiJicantly increased in the Pl compared with P0, P2, P0(R) (p<0.05) and did not significantly differerent u,ith the P3 ), P1(R), Ps(R) (p>0,0s). In this study showed that administration of honey could not improve the levels of ALT and AST as well as abnormal changed macroscopicly and histopathological of male mice liver had been administrated Rhodamin B