Pengaruh Ekstrak Etanol Daun Inai (Lawsonia inermis Linn.) terhadap Aktivitas Superoksida Dismutase dan Histopatologi Pankreas Tikus Wistar (Rattus norvegicus) Hiperglikemia

Abstract

Background : Alloxan can cause experimental diabetic conditions (hyperglycemia) in animal models through oxidation and the fonnation of free radicals ( oxidative stress) which damages pancreatic ~ cells. The ethanol extract of the henna leaves can decrease oxidative stress. This study aimed to determine the effect of ethanol extract of henna leaves on decreasing blood sugar content, increasing the activity of superoxide dismutase enzyme and histopathology image of pancreatic wistar rats induced alloxan. Method : This was an experimental study with posttest only control group design, using rat (Rattus norvegicus) strains wistar which randomized in five groups: negative control group, positive control, group which got ethanol extract group of 200mg/kgbb, 400mg/kgbb and 600mg/kgbb. Results : This research showed that there was significant decrease in blood sugar, compared with the control group. There was activity of superoxide dismutase enzyme was increased but statistically insignificant. Observation of pancreatic histopathology showed a change in diameter in the group receiving ethanol extract of the leaf. Conclusion : The conciusion of the research was that ethanoi extract of the henna leaves 400mg/kgbb decrease blood glucose content of rat significantly, there was no significant difference the increase in the activity of superoxide dismutase enzyme and there was a difference of diameter of langerhans island in the group receiving ethanol extract of the henna leaves 400mg / kgbb.