Isolasi Senyawa Flavonoida dari Kulit Buah Tumbuhan Jengkol (Pithecollobium Lobatum Benth.)

Abstract

Flavonoid compound was isolated from the skin of fruit Jengkol crop (Pithecollobium lobatum Benth.) by using maceration technique with solvent of methanol. Methanol extract that gained from the maceration was concentrated and partition extracted with n-hexane. Methanol fraction was dissolved with acetone repeteadly. Acetone solution was concentrated and put into column chromatography, elucidated with mobile phase CHCl3 : MeOH (80 : 20)v/v and stationary phase is Silica gel 40 (70-230 mesh) ASTM. The Compound was purified like gum form and gained about 185 mg . The Compound was analised by using Spectroscopy Ultraviolet-Visible (UV-Vis), Infra Red (FT-IR), and Nuclear Magnetic Resonance Proton (1H-NMR). Data from the spectrum showed that the compound could be considered one of the flavonoid compound.