Kultur Jaringan Daun Andaliman (Zanthoxylum Acanthopodium Dc.) dengan Perlakuan Ems (Ethyl Methana Sulphonate)

Abstract

The study on ” The Leaf Tissue Culture of andaliman (Zanthoxylum acanthopodium DC.) with induction of EMS (Ethyl Methana Sulphonate)” has been done in Plant Tissue Culture Laboratory of Biologi Department at Faculty of Mathematics and Natural Science North Sumatera University from March until October 2009. The objective of the research was to obtain the effect of concentration and submertion time of EMS to increase growth the leaf culture. The design of the research is complete rendomized design factorial with 2 factors, those are 5 levels of EMS 0,0; 0,05; 0,10; 0,15; and 0,20%, combined with time of submertion of 30, 60, and 90 minutes. The statistic analysis showed that the raising of concentration anda time of submertion of EMS caused decrease of calli fresh weight. The higgest of fresh weight found in C1T2 (0,05% EMS and 60 minute submertion of time).