Aktivitas Antibakteri Edible Film dari Pati Tapioka yang di Inkorporasi dengan Minyak Atsiri Daun Attarasa [Litsea Cubeba(Lour.) Pers.]

Abstract

Isolation of the attarasa leaves [Litsea cubeba (Lour.) Pers.] essential oil has been research by distillation with stahl instrument and and antibacterial activity test with agar diffusion method. Attarasa oil on concentrations 10 %v/v forming zone of transparent againts bacteria Escherichia coli, Shigella dan Staphylococcus aureus with diameters respectively 7,33; 7,88 and 8,17 mm, while on concentrations15% v/v forming zone on transparent to bacteria Escherichia coli, Shigella dan Staphylococcus aureus with diameters respectively 6,66; 7,33 and 10 mm. edible film has made from cassava starch (4% b/b) with glycerol (1% b/b) as plasticizer and incorporated attarasa leaves essential oil (1,5% v/b) and antibacterial activity test with agar diffusion method. Edible film showed zone of transparent on bacteria culture Escherichia coli (7,8mm), Shigella (7mm) but on bacteria culture Staphylococcus aureus did not show inhibition zone. Film of cassava starch-essential oil application to warp carp fish. Density of isolat cell of bacterium caunted by Standart Plate Count (SPC) method. Perception done on day 1, 3, 5 dan 7 indicating that film of cassava starch-essential oil can inhibition growth of colony bacterium of carp fish which is keep in refrigerator. Universitas Sumatera