Pengaruh Pemberian Vitamin C terhadap Kadar Testosteron, Berat Testis dan Jumlah Sperma Mencit Jantan Dewasa (Mus musculus, L.) yang Dipaparkan Monosodium Glutamate (MSG)

Abstract

MSG causes stress oxidative by formatting free radicals which decrease ascorbic acid in testis. Vitamin C as an antioxidant will neutralize free radicals. Taking vitamin C prevents decreasing testosterone level, testicular weight and sperm count. The aim of this study is to investigate the effect of intake vitamin C to testosterone level, testicular weight and sperm count of adult male mice (Mus musculus, L.) which exposed by MSG. Subject of this study was 25 adult male mice (Mus Musculus, L) strain DD Webster, fertile, ±3 months old, 25-35 gram and divided 5 groups. First group is given 0,3 ml NaCl 0,9% intraperitoneal (ip) for 30 days. Second group is given MSG 4 mg/g BW in 0,3 ml NaCl 0,9% (ip) 15 days, 15 days later is given NaCl. Third group is given MSG for 30 days. Fourth group is given MSG.for 15 days and 15 days later is given 0,2 mg/g BW vitamin C in 0,3 ml aquadest orally. Fiflh group is given MSG for 15 days and 15 days later plus vitamin C. Experimental procedures confirmed to the strict guidelines of institutional ethics committee USU Testosterone level is examined using ELFA (Enzyme Linked Fluorescent Assay) methode, testicular weight is measured using sartorius 2402 balance and sperm count using Improved Neubauer counting chamber. there was no significant difference of testosterone level, testicular weight and sperm count mean (p>0, 05), which means taking MSG and vitamin C combined or separated is not affected the testosterone level, testicular weight and sperm count

Monosodium Glutamate (MSG) menyebabkan stress oksidatif ditandai dengan terbentuknya radikal bebas yang mengakibatkan penurunan kadar asam askorbat dalam testis hal ini mengakibatkan penurunan kadar testosteron, berat testis dan jumlah sperma. Vitamin C sebagai antioksidan akan menetralisir radikal bebas. Pemberian vitamin C mencegah penurunan kadar testosteron, berat testis dan jumlah spenna. Tujuan penelitian untuk mengetahui bagaimana pengaruh pemberian vitamin C terhadap kadar testosteron, berat testis dan jumlah spenna mencit jantan dewasa (Mus musculus, L.) yang diberikan MSG. Subjek penelitian mencit jantan dewasa (Mus musculus, L.) strain DD Webster, fertil, ± 3 bulan, 25-35 gram, 25 ekor dibagi 5 kelompok masing-masing 5 ekor. Kelompok pertama diberi NaCl 0,9 % 0,3 ml intra peritonal (ip) 30 hari, kelompok ke-dua diberi MSG 4 mg/g bb dalam 0,3 ml NaCl 0,9% (ip) 15 hari pertama, dan 15 hari berikutnya diberi NaCl, kelompok ke-tiga diberi MSG 30 hari, kelompok keempat diberi MSG 15 hari pertama dan 15 hari berikutnya vitamin C 0,2 mg/g bb dalam 0,3 ml aquadest oral, kelompok ke-lima diberi MSG 15 hari pertama dan 15 hari berikutnya ditambah vitamin C. Penelitian mendapat persetujuan komite etik penelitian USU. Pemeriksaan kadar testosteron menggunakan metode ELFA (Enzyme Linked Fluorescent Assay), berat testis menggunakan timbangan sartorius 2402 danjumlah sperma menggunakan kamar hitung Improved Neubauer. Dari hasil diperoleh perbedaan rata-rata jumlah kadar testosteron, berat testis dan jumlah sperma pada tiap kelompok perlakuan tetapi perbedaan tidak bermakna (p>0,05), ini menunjukkan pemberian MSG dan vitamin C secara sendiri-sendiri maupun bersamaan tidak mempengaruhi kadar testosteron, berat testis dan jumlah sperma