Aktivitas Antimikroba Ekstrak Etanol Batang dan Daun Evodia (Euodia ridleyi Horch.) terhadap Pertumbuhan Streptococcus mutans, Shigella dysenteriae dan Candida albicans secara in Vitro

Abstract

In vitro antimicrobial activity of ethanol stems and leaves extract of Evodia (Euodia ridleyi Horch.) has been conducted. The purpose is to study component of secondary metabolites stems and leaves extract of Euodia ridleyi, antimicrobial activity of the extract against S. mutans, S. dysenteriae and C. albicans and minimum inhibitory concentration against these microorganisms. This research was conducted using extract concentration 0, 5, 10, 20, and 40%. DMSO used as negative control, chloramphenicol and nystatin used as positive control. Extract of Euodia ridleyi were made maceration method and antimicrobial activity was tested by disc diffusion method. The results showed that stems and leaves of Euodia ridleyi Horch. contains alkaloid, flavonoid, steroid, terpenoid, and saponin. Both stems and leaves inhibits the growth of S. mutans, S. dysenteriae, and C. albicans. Minimum inhibitory concentration of ethanol extract stems of Euodia ridleyi against S. mutans and S. dysenteriae is 2%, whereas for C. albicans is 4%. Minimum inhibitory concentrations of ethanol extract leaves of Euodia ridleyi against S. mutans, S. dysenteriae, and C. albicans is 2, 5 and 3% respectively.