Pengaruh Pemberian Vitamin D terhadap Kecepatan Konversi Sputum Pasien TB Paru Etnis Batak dan Gambaran Polimorfisme Foki

Abstract

Background: Vitamin D metabolite helps macrophage phacocyte activity in killing Mycobacterium tuberculosis by increasing intracellular cathelicidin level. This research was aimed to identify the effect of Vitamin D supplementation and Vitamin D Receptor gene Fokl polymorphism in Batak with pulmonary TB. Methods: The design of study was single-blinded randomized control clinical trial, with a total of 92 subject. Inclusion was Batak's people with new cases pulmonary TB having positive sputum smear. Every subject followed and examined for levels of vitamin D at the beginning and at the end of the eighth week, either given a capsule of 100,DOO IU of vitamin D as much as 4x or placebo every 2 weeks. Serial sputum examination was conducted every two weeks and subject were examined for FokI polymorphism by using PCR-RFLP technique Kesimpulan: Vitamin D mempercepat terjadinya konversi sputum dan genotip FF meningkatkan kemampuan ini. Results: Sputum conversion achieved faster m intervention arm than the placebo (p = 0,000). Sputum conversion mean time was 3,95 ± 1,95 weeks in intervention arm and 5,52 ± 2,11 in placebo arm. In the intervention ann, subject with FF has faster conve!'sion time than Ff (p = 0,011). Conclusion: Vitamin D fasten sputum conversion time and FF genotype increase this ability