Aktivitas Antibakteri Ekstrak Etanol dan Fraksi Daun Morbesi-Besi (Tarenna polycarpa (Miq.) Koord. ex Valenton) terhadap Beberapa Bakteri

Abstract

Morbesi-besi leave from the tribe is a kind of tea plant Rubiaceae. Mulberry-leave steel is widely used as antidiabetic drugs, cholesterol, anti-inflammatory and antibacterial. Morbesi-besi leaves contain chemical compounds such as flavonoids, glycosides, steroids / triterpenoids, anthraquinone, saponins and tannins. This study aims to determine the antibacterial activity of ethanol extract and fractions morbesi-iron leaves against some bacterial. Phytochemical screening test done on the powder botanicals, extracts ethanol, n-hexane fraction, the fraction of ethyl acetate and the remaining fraction. Antibacterial activity test was performed using the agar diffusion method to measure the diameter of the clear zone around the paper discs. Testing fraction of n-hexane and ethylacetate performed by TLC using silica gel GF254. He test results of phytochemical screening on botanicals and ethanol extracts are generating the steroid / triterpenoid, glycosides, flavonoids, saponins, antarquinon and tannins, the results of a screening test on a fraction of n-hexane produce triterpenoids / steroids, the results of a screening test on a fraction of ethyl acetate resulted in flavonoids, glycosides, saponins, tannins and screening results in the remaining fraction produces saponins and tannins. Antibacterial activity test results showed inhibition of the Gram-positive and Gram-negative. Ethanol and ethyl acetate extract fractions have the highest antibaketri activity inhibitory concentration of 500 mg / ml inhibitory diameter of 26.8 mm for the ethanol extract of the bacterium Salmonella. typhi, and 27.4 mm in the ethyl acetate fraction for Sthaphylococcus aureus bacteria, while at the :fraction of the n hexane and the remaining fraction showed inhibition were not high as the ethanol extract fraction dab ethylacetate. Diameter resistor in n-hexane fraction of 22. 7 mm in bacterial. Antibacterial activity test results showed inhibition of the Gram positive and Gram-negative. Ethanol and ethyl acetate extract fractions have the highest antibaketri activity inhibitory concentration of 500 mg / ml inhibitory diameter of 26.8 mm for the ethanol extract of the bacterium Salmonella typhi, and 27.4 mm in the ethyl acetate fraction for Sthaphylococcus aureus bacteria, while at the fraction of the n hexane and the remaining fraction showed inhibition were not high as the ethanol extract fraction dab ethylacetate. Diameter resistor in n-hexane fraction of 22. 7 mm in bacteria. on Staphylococcus epidermidis and the remaining fraction has a diameter of 12.4 mm on the inhibition of bacterial acne Propianebacterium. Results 1LC of n-hexane fraction stain visually memunjukkan 5, 9 stain by spraying H2SO4 and 7 stain by spraying Liebermann - Burchard, a compound contained in the n-hexane fraction antibacterial activity is suspected steroids and triterpenoid.