Efek Proteksi Kitosan Terhadap Lambung dan Limpa Tikus Putih (Rattus norvegicus) yang dipapar Plumbum Asetat

Abstract

The plumbum pollution is one of environment pollution in every country. The plumbum in the body will affect to the spleen and gastric. Chitosan’ is an antioxidant that can reduce or absorb heavy metals, so the purpose of research was determine the effect of chitosan on weight and histological of rat’s gastric, spleen and the level of plumbum of rat's gastric that had exposed by Pb-acetate for 7 weeks. The research has been done from January until June 2013 with complete random design (RAL), which consist of seven (7) treatments (K= control; Pi= Pb-acetate 40 mg; P2= Pb-acetate 40 mg and chitosan 0,5% for 5 least weeks; P3~ Pb-acetate 40 mg and chitosan 0,75% for 5 least weeks; P: Pb-acetate 40 mg and chitosan 1% for 5 least weeks; PS= chitosan 1%; Pt acetate acid 1%) which each treatment had 5 replications of rat. The administration of Pb-acetate (40 mg/kgBW/day) is 0.5 ml and the concentration of chitosan is 0,5%, 0,75% and 1% orally using a gavage needle. The result of research showed gastric weight of rat had no significant effect (p>0.05) in each treatment. The result of spleen weight of rat in the treatment of P1-PS increased spleen weight was significantly (p <0.05) to control (K). The results of the level of plumbum of rat’s gastric on PI-P4 significant effect (p <0.05) to control (K). The administration of Ph-acetate affects the histological of rat’s gastric and spleen that is gastric ulcer (P1 and P2) and increasing number of splenic white pulp (PI, P2 and P3), but the administration of chitosan affects the histological of rat's gastric (P3 and P4) and the histological of rat's spleen (P4) as protection in chelating Pb-acetate.