Penetapan Kadar Natrium Siklamat pada Sirup Markisa Madan Secara Kromatografi Cair Kinerja Tinggi (KCKT)

Abstract

Passion fruit syrup is one of the drink product that is synonymous with many outstanding sweet flavor and consumed by the public . In the manufacturing process , manufacturers often use artificial sweeteners to replace the natural sugars to lower production costs . However , in one study , some artificial sweeteners can cause bladder cancer in rats in laboratory experiments , one of which is cyclamate . It is an artificial sweetener that is most commonly used . This study purposed to see if the passion fruit syrup , both local and national products on the market meet the quality standards or not . This study uses high performance liquid chromatography ( HPLC ) , the experimental results obtained by column : oktadesilsilana , 5 lm , 250 mm x 4 mm RP 18 ; fosfat/KH2PO4 mobile phase buffer pH 4.5 and methanol ratio of 7:3 ; flow rate : 1 ml / min ; injection volume : 20 mL ; and detector : UV - VIS 200 nm . From the research , cyclamate levels obtained in passion fruit syrup is 657 mg / kg . According to ISO limit of safe consumption of cyclamate in syrup before diluted is 1000 mg / kg and after diluted at 6000 mg / kg , so it can be concluded that cyclamate levels meet established quality standards .