Efek Peningkatan Dosis Celecoxib dalam Upaya Pencegahan Adhesi Intraperitoneum Pascalaparotomi pada Tikus

Abstract

Intraperitoneal adhesions post laparotomy may increase morbidity and mortality. In order to prevent the formation of intraperitoneal adhesion one of them is by administering anti-inflamatory drug.

To determine the effect COX-2 specific inhibitor celecoxib given in difference doses the incidence of post laparotomy intraperitoneal adhesion.

‘Twenty Sprague Dawley rats (weighing: 150-200 gram) were divided into 4 group (n=5). They were treated with aquadest (1 ml, group I, placebo), diclofenac (1 mg/kgbw; group II), celecoxib low doses (1.4 mg/kgbw; group III), and celecoxib high doses (7 mg/kgbw). Each drug was orally administered once daily for consecutive 3 days. On day 10, each rat was executed to re-laparotomy to determine the incidence the number and the volume of adhesions, and the present of angiogenesis. The data observed were analyzed by one way ANOVA with the level of P<0.05.

The result of study showed intraperitoneal adhesions were observed in all animals of group | and IV (5/5), but the incidence of adhesions were much less in group II (2/5) and group III (1/5). The average of the number intraperitoneal adhesions formed in group I (1.20±0.45) and group IV (1.40±0.55), were not statistically significant. The similar evidence was also occurred between group II (0.40±0.45) and group III (0.20±0.55). The average of the volume intraperitoneal adhesions formed in group IV (0.53±0.22 ml) and group I (0.80±0.31 ml), were not statistically significant. The similar evidence was also occurred between group III (0.06±0.13 ml) and group Il (0.17±0.27 ml). The average of present angiogene: group IV (15.60±7.57) with group I (8.20±7.16), were not statistically significant, The similar evidence was also occurred between on group III (0.60±1.34) and group II (2.60±3,97).

The present demonstrated that the low dose celecoxib (1.4 mg/kgbw) and diclofenac (1 mg/kgbw) could prevent the present of intraperitoneal adhesion and angiogenesis post laparotomy. But, if the dose of celecoxib (7 mg/kgbw) increased of the incidence of the number and the volume of adhesion and the present of angiogenesis was also increased.