| dc.description.abstract | The purpose of this study is to produce 1,3-Propanediol from fermentation of
glycerol as a byproduct of biodiesel production using Enterobacter aerogenes.
This study was done at the Laboratory of Bioprocess Research Center of Oil
Palm, Medan. This study was began with a purification of crude glycerol to
produce pure glycerol and the results of purification were analyzed. And the
process was continued with culture of bacteria for fermentation. Fermentation of
glycerol was done with volume of glycerol was setted 50 ml where variables such
fermentation time (1, 2, and 3 days), volume of inoculum (5, 7, and 10 % from the
volume of glycerol was fermented) and temperature of fermentation (25 and
37◦C) were studied. The results of fermentation were analyzed using Gas
Chromatography (GC). The results of glycerol was obtained that the color of
glycerol was clear, the density of glycerol was 1,2777 g / ml, FFA was 7,83 %,
moisture content was 1,298 %, ash content was 6 % and level of glycerol was
83,2572 %. Analysis of GC showed the purity of 1,3-Propanediol from
fermentation of glycerol, and then the concentration of 1,3-Propanediol was
calculated from that data. The best results of fermentation was obtained in
fermentation 1 day with concentration of 1,3-Propanediol was 6,196 mol/ml, ratio
inoculum : substrate 0,07 with concentration of 1,3-Propanediol was 4,251
mol/ml, and temperature 25 ◦C with concentration of 1,3-Propanediol was 6,433
mol/ml. | en_US |
