Fermentasi Gliserol Hasil Samping Pabrik Biodisel Menjadi 1,3-Propanadiol dengan Menggunakan Bakteri Klebsiella Pneumoniae

Abstract

Oneof the oldestproductsofglycerolfermentation is 1,3-Propanediol. Inthis study, 1,3- Propanediol is producedby fermentation ofglycerolwhichis the result ofpurificationof crudeglycerolfrombiodiesel plants. Cruedglycerolandglycerolof purificationanalyzedthe content ofcompound. Glycerolis fermentedbyklebsiellapneumoniae. Glycerolis fermentedwiththe addition ofvarying thevolume ofbacteriais5,7, and10% (v/v), the fermentation timeis1, 2and3days, and thefermentationtemperatureis 25and37o C. The resultsoffermentationof glycerolwas analyzed usinggas chromatographywithcolumnDB5HTtoobtain apurityof1,3- Propanediol is generatedbased onretention timeofthe standard solution. Crudeglycerolhas areddishbrowncolor, density is1.24g/ml, free fatty acid is26.22%, moisture andash content are2.64 and 11%,and apurityis29.306%. Theresults ofthe purification ofcrudeglycerolhas aclear color,density is1.2676g/ml, free fatty acid is 0.92%, moisture andash content are1.265 and 6%,and apurityis93.1924%. Retentiontime of 1,3-Propanediol is 4.846minuteongas chromatographic analysis. The consentration of1,3-Propanediol at1, 2and3daysis14.0963; 14.6330; and17.6093mol/ml. The consentration of1,3-Propanedioltheaddition ofbacterialvolume5, 7, and10% (v/v) is15.0952; 17.3287; and17.6093 mol/ml. Theconsentration of1,3-Propanediol at 25and37o Cis14.9892and17.6093mol/ml.