Asosiasi antara Alel Gen HLA-DRB1 dan HLA-DQB1 dengan Kerentanan Timbulnya Karsinoma Nasofaring pada Suku Batak

Abstract

HIA gene is suspected to be have a role in the development of

nasopharyngeal carcinoma (NPC). To assess the relation between HLA- DRBl dan HLA-DQB1 alleles with NPC among the Batak people, this case- control study was performed using samples consisted of 55 case and 104

controls. DNA isolation was conducted at the Laboratorium Terpadu of Universitas Sumatera Utara, while gene allele identification was carried out using PCR-SSO reverse dot blot method at The Department of lmmunohematology and Blood Transfusion, Leiden University Medical Center. The majority of NPC sufferers were males (60%), 50-59 years old (29,090%), and work as farmer (36.363%). Histopathologically, the majority was WHO Type 3 (54.545%), followed byWHO Type 1 (29.091%) and WHO Type 2 (16.364%). Most of them were in Stage lll (67.273%), followed by Stage lV (25.455o/o) and Stage ll (7.273o/o). There was a significant relationship between daity consumption of satted fish before the age of 10 years old with NPC (p<0.05), OR 6.029 (Cl 2.900-12.538). The shortest genetic distance was found between the Batak people with the Kalha Mongolia tribe (D=0.291), compared to the population of Man of North China and South Koreans. Highest frequency of allele gene of Batak population etnic was HLA-DRB1.12 (71.154Yo) and HI-A-DQB1"0301 (75.962%), and

among the NPC sufferers it was H1A-DR81.12 (63.363%) and HLA- DQB1.0301 (70.909%). HLA-DRB1.08 allele gene was significantly

associated with NPC (p<0.05), OR 4,122 (Cl. 0.989 - 17.180). This study concludes that HIA-DRB1.08 allele gene has a potential in the susceptibility for NPC among the Batak people