Aktivitas Antibakteri Isolat Bakteri Asam Laktat dari Terasi Udang Rebon Asal Kecamatan Medan Belawan terhadap Bakteri Patogen

Abstract

Shrimp paste as a food flavoring ingredient by the people of Indonesia is a product of fermented fish or shrimp that undergoes salting treatment. High use of salt in the fermentation process will cause the growth of lactic acid bacteria. Improper handling of processed products results in fishery processed products being easily contaminated by pathogenic bacteria such as Escherchia coli and Staphylococus aureus. Control of putrefactive and pathogenic bacteria in fermentation can be carried out biologically by the addition of antibacterial substances derived from metabolites of lactic acid bacteria (BAL) that are able to inhibit the growth of contaminant bacteria This research conducts a test of the inhibition of pathogenic bacteria using the well diffusion method using Mueller Hilton Agar media. (MHA) and observed for 5 days. This study aims to determine the morphological and biochemical characteristics of BAL shrimp paste from Medan Belawan District and BAL antibacterial activity of rebon shrimp paste against pathogenic bacteria Escherchia coli and Staphylococus aureus. From the results of testing morphological characteristics obtained isolates having the shape of a spherical colony (Circular), the elevation of the convex colony (Convex), the edge of the colony flat (Entire), the color of the colony is milky white and yellowish-white. Microcopically has the shape of stem cells (Bacillus) and round (Coccus), Gram positive and negative and the biochemical characteristics obtained have positive motility test results, negative catalase results, Methyl Red 4 positive isolates and 6 negative isolates, Voges Proskeur negative and TSIA tests consisting of K / A / ; K/K; A / A which also exists contains gas and H2 S and does not contain gas and H2S. Results showed that the antibacterial activity of BAL shrimp paste rebon against Escherchia coli with a diameter of 20 mm is classified as strong and against Staphylococus aureus with a diameter of more than 20 mm is classified as very strong.