Hubungan Ekspresi Imunohistokimia Matriks Metalloproteinase-9 ( MMP-9 ) dengan Karakteristik Klinikopatologi pada Colorectal Adenocarcinoma Not Otherwise Specified (NOS)

Abstract

Background:Matrix metalloproteinase-9 (MMP-9) is a member of metalloproteinase enzymes that are often found in colorectal adenocarcinoma. MMP-9 is able to destroy basement membrane and extracellular matrix proteins that play a role in tumor cell invasion. Destination: This study was conducted to analyze the relationship between MMP- 9 expression and the clinicopathological characteristics of colorectal adenocarcinoma NOS. Method:The study used 30 paraffin block samples of adenocarcinoma colorectal NOS cases that had been diagnosed at the Laboratory of Anatomic Pathology, H. Adam Malik Hospital, Medan and at the Department of Anatomic Pathology, Faculty of Medicine, University of North Sumatra. MMP-9 expression was assessed using immunohistochemical staining of the cytoplasm of tumor cells and cells of the stroma. Expressions were assessed using the Quick Score method and were declared weak when the total score was 0-5 and strong when the total score was 6. Statistical analysis between MMP-9 expression and the depth of invasion was performed using Fisher's Exact and Mann-Whitney U tests. Results:MMP-9 expression was significantly related to stage (p=0.01), depth of invasion (p=0.03), and stromal Tils (p=0.03) and not significantly related to gender (p=0.28). , age (p=0,82), tumor location (p=0,69), grading (p=0,26), lymph node involvement (p=0,53), metastasis (p=0,53), perineural invasion (p=0.25), lymphatic invasion (p=1.00), IMVI (p=0.531.00), EMVI (p=1.00), tumor budding (0.25) Conclusion :MMP-9 expression can be used as a prognostic indication and also helps clinicians, especially in the management of patients with colorectal adenocarcinoma NOS.