Isolasi dan Penentuan Struktur Kimia Senyawa Flavonoid dari Daun Iler (Coleus atropurpureus Benth)

Abstract

Iler ( Coleus atropurpureus Benth) is a species of the family Lamiaceae which is used as a traditional medicine to treat fever, ulcer wound and diabetes mellitus. This plant is known to contain flavonoid. This study aimed to isolate and determine the molecular structure of flavonoid compounds from the leaves of C. atropurpureus Benth. Extraction was carried out by maceration using methanol as solvent and the methanol extract was partitioned with n-hexane solvent. Ethylacetate was added to the methanol dried extract and then filtered, the ethylacetate extract were separated into its components using column chromatography with silica gel 60 as stationary phase and n-hexane-ethylacetate (6:4 v/v) as mobile phase. A pure isolate was obtained as a yellowish amorphous solid (37 .5 mg). The structure of the pure isolate were determined by spectral analysis, i.e. UV-Vis, FT-IR, ID- and 2D-NMR and LC-MS experiments. Based on spectral analysis that the isolate was concluded to be a flavonoid compound of flavone type namely apigenin-7 -O-glucoside.