Pemanfaatan Acetobacter xylinum Untuk Pembuatan Material Selulosa Bakteri Kitosan dari Maltosa dibandingkan Sukrosa Menggunakan Medium Air Kelapa

Abstract

One of important coconut water application is for preparation of nata de coco or bacterial cellulose. The resulting bacterial cellulose in fact can be used in medical application as temporary substitute for skin or wound cover, and the product through fermentation process of coconut water can be improved by addition of carbohydtate compound as source of glucose into prepared fermentation medium. In this research, glucose of sucrose was used, and maltosa continued cabrohydrate was added to form bacterial cytosan cellulose material whose application is expected to render medical application with multiple properties. By using 100 mL of coconut water being administered 0,5 g of urea and 10 g of sucrose or maltosa and followed by addition of acetat acid until pH 4, the fermentation was then made by using Acetobacter xylinum to produce the bacterial cellulose. Furthermore, each fermentation medium could produce material of cytosan bacteri cellulose cytosan cellulose by addition of cytosan in mass variations 0,5 g, 1,0 g, 1,5 g, 2,0 g for 12 hours. The result gained is pellicle layer floating on medium surface. And then the pellicle was dried out, and thin layer was formed as bacteri cellulose or bacteri cellulose or bacterial cytosan cellulose material. From the result of SEM, FT-IR and pulling analysis test, the best treatment result was variable by addition of cytosan for 0,5 g of sucrose, the ductile strength was 20,38 Mpa, and flexibility was 29,03 %, however by using raw material of maltosa in treatment the best result was variable by addition of 0,5 g maltosa, tensile strength was 9,60 Mpa, and flexibility was 48,38 %.