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    Aktivitas Antikanker Ekstrak Etanol dan Fraksi Daun Ekor Naga (Rhaphidophora pinnata (L-f). Schott) terhadap Set MCF-7 dan Isolasi Senyawa Kimia Aktif sebagai Antikanker

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    Date
    2014
    Author
    Masfria, Masfria
    Advisor(s)
    Harahap, Urip
    Nasution, Martua Pandapotan
    Ilyas, Syafruddin
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    Abstract
    Indonesia is rich in medicinal plants, one of which that was believed to be effective against cancer is “ekor naga” leaf (Rhaphidophora pinnata (L.f)Schott). The boiled “ekor naga” was traditionally used for the treatment of east cancer, and from the previorus study by brine shrimp (Artemia salina Leach) test showed that ethanolic extract of “ekor naga” leaf (EDEN) showed cytotoxic activity. The objective of this study is to determine the cytotoxic activity of FEDEN, the fractions and the isolate of ekor naga leaf againt MCF-7 cancer cells. Antiproliferation and apoptosis assay had been conducted for cytotoxic isolate, followed by the isolation of active compound. Characterization of crude drug and phytochemical screening were performed on the dried powdered crude drug and the ethanolic extrat of “ekor naga” leaf (EEDEN), the phytochemical screening also performed on the n- hexane, chloroform, ethylacetate, and aqueous fractions. The ekor naga leaf was extracted. by percolation using ethanol as solvent, followed by fractionation with Tiguid-liquid extraction using n-hexane, chloroform, and ethylacetate as solvents. The cytotoxic activity testing was performed for the fractions (n-hexane, chloroform, ethyl acetate, and aqueous) against MCF-7 cells with MIT assay (3 (4.5-dimethyltiazol-2-e))2,5-diphenyltetrazoliumbromide) The active chloroform fraction as anticancer was subjected to column chromatography: the resulting isolate was tested for its cytotoxicity activity. The isolate D and F from fractionation were tested for its antiproiferative (doubling time) with observation time 24; 48 and 72 hours using MTT assay. The isolate D was tested for apoptosis induction by double staining assay using acridine orange-ethidium bromide and by floweytometry assay using Ancksin V against the MCF - 7 cells. The result of characterization of dried powdered ekor naga leave showed brownish colour, odorless, tasteless powder; the water content was 8.99%; water soluble extract 14.81% ethanol soluble extract 9.76%, total ash content 10.09%, and acid insoluble ash 0.17%. Phytochemical screening of dried powdered ckor naga leaves and EEDEN showed the presence of tannin, alkaloid, flavonoid, saponin, glycoside, and triterpenoid/steroid compounds; the n-hexane fraction gave 2 positive result for triterpenoid/steroid; the chloroform fraction showed a positive result for alkaloid, and triterpenoid/steroid; the ethylacetate fraction showed a positive result for flavonoid, tannin, and glycoside, while the aqueous fraction contained tannin, saponin, and glycoside. Probit analysis gave ICsp values, for EEDEN, chloroform fractions and ethyl acetate fractions were 112.240; 59,082; 812.663 ug/mL, respectively while the n-hexane and aqueous fractions did not inhibit the growth of MCF-7 cells The result of the cytotoxic activity testing for A through I isolates showed IC50 values of 190.550; 134.014; 204.474; 87.831; 96.016; 34.161; 9.6215, 758.572; 245.446 uglml. respectively. The doubling time result of D and F isolates could inhibit cell proliferation depending on the concentration, aliquot with incubation time the ICs value decreased. at 72 hours observation. Apoptosi observation for isolate D and isolate F used floweytomerry assay also showed apoptosis and necrosis. The separation of fraction F by column chromatography gave six isolates with FFI and FF2 showed one TLC spot which then characterized by UV, FTIR and LC-MS methods. The UV spectrum of FFI isolate had maximum absorption peak at % 238 and 402 nm while FF2 isolate at 2 of 408 nm. The inftared spectrum showed the presence of OH, -CH aliphatic (CHs and ~CHs) C C and C = 0 groups. MS data for FF1 isolates showed fragmen peak mie 429 and 319 while FF2 isolate showed fragmen m/e 466.6 that suggested to be steroidtritepenoid, Column chromatography separation of isolate A resulted five isolates with FA: was then purified by column chromatography to give FA2.22 isolate The PA2.2.2 isolate was characterized by UV spectrophotometry, FT-IR, GC-MS and NMR. The infrared spectrum showed. the presence of -OH, -CH aliphatic CH: and -CHh), C= C and C = O groups. The "C-NMR data showed 23 carbon toms, GC-MS detected seven compounds, with four of them had similarity indices greater than 90%,
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    https://repositori.usu.ac.id/handle/123456789/81496
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    Repositori Institusi Universitas Sumatera Utara (RI-USU)
    Universitas Sumatera Utara | Perpustakaan | Resource Guide | Katalog Perpustakaan
    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV