Isolasi dan Identifikasi Golongan Senyawa Fenolik dari Kulit Batang Tumbuhan Delima (Punica granatum L.)

Abstract

The phenolic compound had been isolated from the stem bark of pomegranate (Punica granatum L.). Isolation was carried out by maceration extraction method with methanol and methanol extract was re-extracted with ethyl acetate repeatedly untul negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform: methanol (90:10; 80:20; 70:30; 60:40) v/v . Fractions 86-160 was purified by preparatif thin layer chromatography using chloroform : methanol (80:20) v/v eluent to produce a greenish yellow amorphous solid of 7,3 mg at Rf value of 0,6 using chloroform:methanol (80:20) v/v as eluent and Rf 0,66 using chloroform : ethyl acetate (20:80) v/v eluent. Based on the analysis of the UV-Visible spectrophotometer, it was a wavelength (λ max) of 275 nm, the FT-IR spectrum shows the presence of OH, C=C aromatic, C=O ester, C-H, and C-O groups. The proton Nucleus Magnetic Resonance Spectrum (1H-NMR) shows the presence of H-2, H-6 protons, proton from CH3 bins to the benzene ring, and methoxy protons. Based on the data analysis and interpretation, the isolated compound was a phenolic compound derived from phenolic acid.