| dc.description.abstract | The phenolic compound had been isolated from the stem bark of pomegranate (Punica granatum L.). Isolation was carried out by maceration extraction method with methanol and methanol extract was re-extracted with ethyl acetate repeatedly untul negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform: methanol (90:10; 80:20; 70:30; 60:40) v/v . Fractions 86-160 was purified by preparatif thin layer chromatography using chloroform : methanol (80:20) v/v eluent to produce a greenish yellow amorphous solid of 7,3 mg at Rf value of 0,6 using chloroform:methanol (80:20) v/v as eluent and Rf 0,66 using chloroform : ethyl acetate (20:80) v/v eluent. Based on the analysis of the UV-Visible spectrophotometer, it was a wavelength (λ max) of 275 nm, the FT-IR spectrum shows the presence of OH, C=C aromatic, C=O ester, C-H, and C-O groups. The proton Nucleus Magnetic Resonance Spectrum (1H-NMR) shows the presence of H-2, H-6 protons, proton from CH3 bins to the benzene ring, and methoxy protons. Based on the data analysis and interpretation, the isolated compound was a phenolic compound derived from phenolic acid. | en_US |
