| dc.description.abstract | Background: Allergic disease is an important issue because it occurs at all
levels of society and increasing the number of events in the last three decades.
Allergy risk factors and cow's milk allergen sensitization together with
environmental factors play an important role in the mechanism of allergy.
Development of allergic diseases is determined by at least three things: the
-genetic background, exposure to allergens and amplified by environmental
factors. Wheezing is an early symptom of respiratory disorders that often occur
as a result of allergic reactions in cliildren. Children· from families witli a history of
allergic diseases will be at risk of allergic disease compared with those without a
history of allergic disease in the family.
Objective: Looking at the role of cow's milk allergen sensitization on the
lymphocytes Th-1 and Th-2 activity and allergy risk in parent with environmental
factors in association with wheezing in infants up to age 1 year were treated in
the General Hospital dr. Zainoel Abidin Banda Aceh.
Methods: This exploratory analytic observational study with cohort design was
conducted at General Hospital dr. Abidin Abidin Banda Aceh from May to
December 2013. Samples were taken with consecutive technique based on a
single sample formula to estimate the proportion of the population using
absolute precision in order to obtain 71 samples that fit the inclusion criteria.
Examination of skin prick test as a diagnostic procedure doing by a pediatrician
division of allergy and immunology, and then taken in the peripheral blood of
infants median cubital vein by 5 ml by the inspection team Prodia for IL-5 and
IFN-y levels by ELISA in laboratory techniques Prodia Jakarta. Samples were
monitored regularly by telephone to determine allergic complaints from
prospective subjects performed every week until the age of 1 year. Univariate
analysis covers to see the distribution of each variable, to test the hypothesis at
the bivariate level 2 variant and look for the point of intersection with the means
of descriptive statistics (mean and median), and multivariate multiple logistic
regression to determine risk factors. To determine the relationship between the
various variables to wheeze using Chi Square test and Fisher's exact test. To
assess the differences in activity of Th-1, Th-2 and the results of skin prick test
- in wheezing and non-wheezing group used independent t test.
Results: A cohort study in 71 subjects were followed for up to 12 months old
obtained the incidence of wheezing was 64.8%. Subjects who have a low risk of
allergic-being as much as 67.6% and a high allergy risk as much as 32.4%. The
bivariate analysis there is significant relationship between IL-5 level with the
result of skin prick test.
There is a relationship of allergy risk factors (p = 0.007), cow's milk skin prick
test (p = 0.000), IFN-y levels (p = 0.033), IL-5 levels (p = 0.000) and the father
smoked (indoor) (p = 0.003) on the complaint of wheezing. While history of labor (p = 0.337), history of feeding (p = 0.232) and sex (p = 0.872) was not
related to the complaints of wheezing. There are differences in the value of the
skin prick test in children with high risk factors and mild-moderate risk factors (p
= 0.010). There are differences in the levels of IL-5 in children with symptoms of
wheezing and non-wheezing (p = 0.000). There are differences in the value of
the skin prick test in children with symptoms of wheezing and non-wheezing (p
= 0.000). But there are differences in levels of IFN-y in children with symptoms
of wheezing and non-wheezing (p = 0.192).
In multivariate analysis contained significantly influence levels of IFN-y against
wheezing (p = 0.010) with RR 10.28 (95% Cl 1.73 to 31.61), the levels of IL-5
against wheezing (p = 0.000) with RR 20 , 69 (95% Cl 4.51 to 94.83) and
patem~I smoking on wheezing (p =_ 0,020) with RR 6.49 (95% _Cl 1.33 to 31.61).
Conclusion: There Js a significant relationship between IL-5 level with ·the
result of skin prick test. Risk factors has significant relation on the incidence of
wheezing. There are difference of average value on skin prick test results in a
group of high risk and mild-moderate risk factors. Mean value of IFN-y did not
differ in wheezing· and non-wheezing group. Mean value of IL-5 differ in
wheezing and non-wheezing group. There are difference of average value on
skin prick test results in wheezing and non-wheezing group. Parents smoking
~ndoor), particularly the father seemed related to the occurrence of wheezing.
There is no relations of labor history and feeding history and sex on the
occurrence of wheezing. The results of this study showed a shift picture of
allergic march in Aceh with early wheezing symptoms in 4-6 month as an early
sign of asthma and dermatitis. The result of study is also showed IL-5 level
increased above 0,6 pg/ml with· AUC is 86% reflected the Th-2 profile
examination is so realibel in allergies determination | en_US |
