| dc.description.abstract | Isolation and identification of groups of phenolic compound contained in the bark of the Kalpataru Plant (Hura crepitans Linn) have been carried out by maceration extraction using methanol as a solvent. The concentrated methanol extract was re-extracted using ethyl acetate repeatedly until it was negative for FeCl3 5%. The ethyl acetate extract was concentrated and redissolved with methanol and then partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform: ethyl acetate (90:10; 80:20; 70:30; 60:40 50:50; 40:60) v/v, respectively. The compound obtained from fraction 131-190 was purified by preparative thin layer chromatography to produce 12 mg yellow amorphous solid with an Rf value of 0,44 with chloroform: methanol (90:10) v/v as the eluent. Analysis of the compounds obtained was carried out using UV-Vis spectrophotometry, FTIR, and 1H-NMR. The UV-Vis spectrum shows a wavelength (λ max) of 217 nm in band II and 275 nm in band I. The FT-IR spectrum shows the presence of –OH stretching, CH stretching, C=O stretching, C=C stretching, bending C-H, C-O stretching, respectively, at wave numbers 3356,14 cm-1 , 2924.09 cm-1, 1693,50 cm-1, 1535,34 cm-1, 1442.75 cm-1, 1002,98 cm-1. The 1H-NMR spectrum shows the presence of protons H-2 and H-6 in the aromatic ring of phenolic compounds, protons H-2' from CH3 and protons H-4 from CH3 attached to the benzene ring and the H-1' of OCH2 and protons H from CH2 which uses on the aromatic ring. Based on the data obtained, it is suspected that the isolated compound obtained is a phenolic compound derived from phenylacetic acid. | en_US |
