| dc.description.abstract | Background: Indonesia has a very large ocean area that provides various sources
of food needed by the community, one of which is the blood clam (Anadara
granosa L.) which can be found in the Indopacific from East Africa to Polynesia,
Japan, Australia and along the coast of India. Blood clam (Anadara granosa L.) is
a type of shellfish that has the potential and high economic value to be developed
as a source of protein and minerals to meet the food needs of the Indonesian people.
Degradation of proteins into peptides by enzymes will increase their biological
activity, in this case bacteria. In addition, the peptide has a smaller size so that it is
easier to enter and penetrate the pores on the bacterial membrane, so it has a higher
antibacterial activity.
Objective: This research was conducted to determine the antibacterial activity of
protein hydrolyzate of blood clams (Anadara granosa L.) using papain enzymes
against Escherichia coli and Staphylococcus aureus bacteria and to determine the
effect of hydrolysis time.
Method: Blood clam samples were obtained from the Medan Belawan market.
Then the hydrolysis process was carried out using the papain enzyme with time
variations of 0 hours, 8 hours, 16 hours and 24 hours. After that the protein
hydrolysate is dried in the oven. Furthermore, the antibacterial activity was tested
by agar diffusion method against Escherichia coli and Staphylococcus aureus.
Results: The results showed that the protein hydrolyzate of blood clams (Anadara
granosa L.) using the papain enzyme had antibacterial activity against Escherichia
coli and Staphylococcus aureus, with a minimum inhibitory concentration of the 0
hour hydrolyzate is 100 μg/ml, the 8 hours hydrolyzate is 100 μg/ml, the 16 hour
hydrolyzate is 50 μg/ml and the 24 hour hydrolyzate is 50 μg/ml. In testing the
antibacterial activity of the protein hydrolyzate of blood clams (Anadara granosa
L.) against Escherichia coli and Staphylococcus aureus it was higher at 24 hour
hydrolyzate, respectively 9.86 ± 0.15 mm and 8.60 ± 0.30 mm at a concentration of
500 g/ml.
Conclusion: The protein hydrolyzate of blood clams (Anadara granosa L.) had
antibacterial activity against Escherichia coli and Staphylococcus aureus and the
antibacterial activity of the hydrolyzate of blood clams (Anadara granosa L.) was
higher at 24 hours hydrolyzate. | en_US |
