Pengaruh Aplikasi Chip Kombinasi Ekstrak Ikan Gabus (Channa Striata) dan Daun Sirih (Piper Betle L) pada Tikus Wistar yang Diinduksi Periodontitis sebagai Penunjang Terapi Inisial : Evaluasi terhadap Parameter Klinis dan Ekspresi TNF-a (In Vivo)

Abstract

Background : Scaling root planing is the main mechanical treatment to remove local deposits. The limitations of conventional therapy in periodontal treatment can be overcome by using antibiotics and antiseptics as supporting therapy. Local antimicrobials such as periodontal chips have a minimum inhibition of more than 99% against periodontal pocket bacteria. The chip application of the combination of snakehead fish extract and betel leaf can change the clinical parameters for the better and the expression of TNF-α as a pro-inflammatory. Objective: To see the effect of the application of a chip combination of snakehead fish extract and betel leaf in male Wistar rats induced by periodontitis as a support for initial therapy on clinical parameters and expression of TNF-α. Methods: Laboratory experimental study with a posttest control group design in which 24 male Wistar rats induced by periodontitis were divided into four treatment groups (periodontal chip extract of snakehead fish 2.5%, 5%, 10% and placebo). Examination of clinical parameters and expression of TNF-α was carried out on day 0, day 3 and day 7 after treatment with rats. Data were analyzed by Mann-Whitney test. Results: The results of this study showed that changes in clinical parameters were better on the 7th day after treatment with statistically significant results (p≤0.05) and the expression of TNF-α with the highest anti-inflammatory occurred at a concentration of 5%. Conclusion: The application of the chip combination of snakehead fish extract and betel leaf can reduce the gingival index and pocket depth, as well as significantly increase the level of attachment and expression of TNF-α. The application of the chip combination of snakehead fish extract and betel leaf with 5% concentration were high on the 3rd nor on the 7th day.