Identifikasi Polimorfisme Gen CYP1A1 (3801TC dan Ile462Val) pada Karsinoma Sel Skuamosa dan Adenokarsinoma Serviks

Abstract

Objectives: To identify the frequency and distribution of CYP1A1 gene polymorphism (3801T/C dan Ile462Val) in squamous cell carcinoma and adenocarcinoma of the cervix. Methods: This cross-sectional descriptive study was conducted in Department of Anatomical Pathology, University of Sumatera Utara and H. Adam Malik Hospital Medan with 46 samples of cervical cancer patients. CYP1A1 gene polymorphism (3801T/C or Ile462Val) was analyzed using PCR-RFLP method followed by gel electrophoresis. Results: CYP1A1 gene polymorphisms (3801TC) in squamous cell carcinoma were 50% heterozygote T/C, 36% wild-types T/T and 14% homozygote C/C. CYP1A1 gene polymorphisms (3801TC) in adenocarcinoma were 60% heterozygote T/C and 40% wild-types T/T. CYP1A1 gene polymorphisms (Ile462Val) in squamous cell carcinoma were 97.2% heterozygote Ile/Val, and 2.8% homozygote Val/Val. CYP1A1 gene polymorphisms (Ile462Val) in adenocarcinoma were 100% heterozygote Ile/Val. Conclusion: The result showed that the most common type of CYP1A1 gene polymorphism (3801TC and Ile462Val) in squamous cell carcinoma were heterozygote T/C and Ile/Val which count 50% and 97.2%, respectively. Meanwhile, the most common type of CYP1A1 gene polymorphism (3801TC and Ile462Val) in adenocarcinoma were heterozygote T/C and Ile/Val which count 60% and 100%, respectively.