| dc.description.abstract | Analysis of cannabinoids in hair of marijuana (Cannabis saliva L) can be
done using samples of urine, blood and hair. This study used hair because the
inspection can be performed after 72 hours of use by the user, this is different to
using urine and blood samples. Based on the previous reference that cannabinoid
compounds were detected in general is 1J9THC compounds and long extraction time
and GCMS techniques. The Objective is to obtain a new method of analyzing
cannabinoid of hair of marijuana (Cannabis saliva L.) abusers using techniques
GCMS and to validate the methods. In this study, Analysis used of the hair marijuana
(Cannabis Saiva L) abusers using GCAMS technique. The hair samples taken from Al
Kamal Rehabilitation Sibolangit Centre. Cannabis plants were collected from
evidence at the Forensic Laboraiory Branch Medan. Extraction used maceration
method with sonication at 42 KHZ and the solven : methanol - 2-propanol (]: 1),
chloroform, benzene and petroleum 2 ml for 5 minutes. The result of extraction
process tested with fast blue salt B test produces a Violet precipitate. The Optimal
results obtained at the extraction temperature of 50°C. Identification followed by
Thin Layer Chromatography (TLC) using the mobile phase toluene and n-hexane (I:
1) under alkaline conditions (pH 9.5). Results extraction followed by GC\1S
technique during the sixteen (16) minutes. The results showed that the hair was
obtained the compound cannabidiol, cannabinol, and ,,J9THC with concentrations
ranging from 0.25 to 2.82 ng/mg. Decreased levels for 30 days of cannabidiol,
cannabinol and 1J9THC average respectively 0.06, 0.02, and 0.03 ng I mg. The
validation of GCMS method was results cannabidiol, cannabinol, and 1J9THC
generate the accuracy (precision) value with the percent recovery (% recovery)
respectively 103.83, 102.67, and 101.17. Results of precision test was RSD =
1.3058%, 0.8997% and ft.8997%. Linearity test: r = 0.922, 0.955, and 0.921. LOD =
0.000168 ng/mg, 0.000117 ng/mg, and 0.000164. Quantization limit LOQ = 0.00056
ng I mg, 0.00039 ng I mg, and 0.00054 ng I mg. Based on the results above,
modification of the extraction process and GCMS technique used can produce
cannabinoid compounds (cannabidiol, cannabinol, and 1J9THC) in hair samples
quickly and accurately. | en_US |
