Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Tumbuhan Antahasi (Weinmannia Blumei Planch)

Abstract

The flavonoid compound had been isolated from the bark of antahasi (Weinmannia blumei Planch). Isolation was carried out by maceration extraction method with methanol and methanol extract was re-extracted with ethyl acetate repeatedly until negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-heksane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and mobile phase of eluent chloroform : methanol (90:10; 80:20; 70:30; 60:40) v/v. The compound obtained from fraction 71-113 was purified by chloroform and ethyl acetate, produced a yellow amorphous solid of 8,7 mg with Rf = 0,44 using chloroform : methanol (80:20) v /v as eluent and Rf = 0,44 using chloroform : ethyl acetate (20:80) v /v as eluent. Based on the analysis of UV-Vis spectrum with methanol solvent which showed wavelength (λmax) 280 nm. FT-IR spectrum showed the presence of OH, stretching sp3 C-H, C=O ketone, C=C aromatic, bending sp3 C-H, CO and COC successively at wave number 3392,79 cm-1, 2927,94 cm-1, 1627,92 cm-1, 1523,76 cm-1, 1379,10 cm-1, 1290,38 cm-1, 1147,65 cm-1. The Proton Nucleus Magnetic Resonance Spectrum ( 1H-NMR) showed the presence of H-5, H-2’, H-6’, H-3’, H-5’, H-6, H-8, H-2 and H-3 protons. Based on the interpretation of spectroscopy data obtained by the isolated compound are flavonoid compound of the flavanone group.