| dc.description.abstract | The flavonoid compound had been isolated from the bark of antahasi (Weinmannia blumei Planch). Isolation was carried out by maceration extraction method with methanol and methanol extract was re-extracted with ethyl acetate repeatedly until negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-heksane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and mobile phase of eluent chloroform : methanol (90:10; 80:20; 70:30; 60:40) v/v. The compound obtained from fraction 71-113 was purified by chloroform and ethyl acetate, produced a yellow amorphous solid of 8,7 mg with Rf = 0,44 using chloroform : methanol (80:20) v /v as eluent and Rf = 0,44 using chloroform : ethyl acetate (20:80) v /v as eluent. Based on the analysis of UV-Vis spectrum with methanol solvent which showed wavelength (λmax) 280 nm. FT-IR spectrum showed the presence of OH, stretching sp3 C-H, C=O ketone, C=C aromatic, bending sp3 C-H, CO and COC successively at wave number 3392,79 cm-1, 2927,94 cm-1, 1627,92 cm-1, 1523,76 cm-1, 1379,10 cm-1, 1290,38 cm-1, 1147,65 cm-1. The Proton Nucleus Magnetic Resonance Spectrum ( 1H-NMR) showed the presence of H-5, H-2’, H-6’, H-3’, H-5’, H-6, H-8, H-2 and H-3 protons. Based on the interpretation of spectroscopy data obtained by the isolated compound are flavonoid compound of the flavanone group. | en_US |
