Isolasi dan Identifikasi Golongan Senyawa Flavonoid dari Daun Tumbuhan Ridan (Nephelium maingayi Hiern)

Abstract

The flavonoid compound had been isolated from the leaves of ridan (Nephelium maingayi Hiern). Isolation was carried out using maceration extraction method with methanol and methanol extract was re-extracted with ethyl acetate repeatedly until negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was separated using column chromatography with a stationary phase of silica gel and a mobile phase of chloroform - methanol (90:10, 80:20, 70:30, 60:40) v/v. The compound obtained from fraction 95-144 was purified by preparative thin layer chromatography and the resulting isolate was 8 mg yellowish-brown amorphous solid with an Rf value of 0.68 with chloroform - methanol (70:30) v/v as eluent. Based on the results UV-Visible analysis, it showed a wavelength (λmax) of 317 in the band I and 289 nm in the band II, the FT-IR spectrum showed the presence of OH, C-H stretching, C = C aromatic, C = O, C-H bending and C-O groups. The Proton Core Magnetic Resonance Spectrum ( 1H-NMR ) showed the presence of H-2’ ; H-6’ ; H-3’ ; H-5’; H-8 and H-6 on the aromatic rings. Based on the data obtained, the isolated compound is a flavonoid compound of the flavone group.