Pengaruh Infusa Kulit Jeruk Nipis (Citrus aurantifolia swingle) terhadap Jumlah Fibroblas pada Proses Penyembuhan Luka Pasca Ekstraksi Gigi pada Tikus Putih (Rattus norvegicus) Strain Wistar

Abstract

Tooth extraction is a procedur of removing the teeth from the alveolar bone. The teeth are extracted leaving the socket consist of the cortex of the bone by periodontal ligament, protected by gingiva as an outer layer. In the process of wound healing needs a substance to accelerate the process of wound healing needs a substance to accelerate the process of wound healing, one of the natural ingredients that can improve the wound healing process is flavonoid contained in lime peel. This research aims to know the effect of the decoction of lime peel (Citrus aurantifolia swingle) towards the number of fibroblast on the wound healing process in white rats (Rattus norvegicus) wistar strain. This study is an in vivo experimental study with post test only control group design with 30 wistar rats (Rattus norvegicus) as the research sample and divided into 6 groups. Treatment group that is used was group of the rats given lime peel decoction after tooth extraction and decapitation on day 7,14 and 21, and the control group that is used was groups of the mice that were not given the lime peel decoction post tooth extraction and decapitation on day 7,14, and 21. The specimens were then stained with Hematoxylin Eosin (HE) staining to observe changes in fibroblast cells that occured on the 7th, 14th, and 21st days. Based on parametric independent T test showed that there was effect of infusion lime peel towards the number of fibroblast on the wound healing process in white rats wistar strain on days 7 with p value 0,030 (p<0,05), days 14 with p value 0,040 (p<0,05), days 21 with p value 0,0001 (p<0,05). Giving infusion of lime peel can increase the average number of fibroblast cells on the wound healing process in white rats wistar strain.