Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Daun Tumbuhan Mahang Damar (Macaranga triloba (Thumb) Mull. Arg)

Abstract

The flavonoid compound had been isolated from the leaves of (Macaranga triloba (Thumb.) Mull.Arg). Isolation was carried out using maceration extraction method with methanol. Methanol extract was re-extracted with ethyl acetate repeatedly until negative to 5% FeCl3.The Ethyl acetate extract was evaporated until solid and partitioned with n-hexane until the n-hexane layer was clear, the methanol layer is separated from the n-hexane layer. The methanol layer was evaporated until solid. The methanol solid extract was separated by column chromatography with silica gel 60 as the stationary phase and chloroform - methanol as the eluent as mobile phase (90:10; 80:20; 70:30; 60:40)v/v. Purification was carried out on fraction 186-271 by preparative thin layer chromatography and resulted in 8 mg of isolate in the form of a yellowish brown amorphous solid with a value of Rf 0.82 with chloroform - methanol (80:20)v/v eluent. The UV-Visible spectrum of the isolate shows a wavelength absorption (λmax) of 362 nm in band I and 255 nm in band II. The FT-IR spectrum of the isolates showed the presence of OH groups, symmetric and asymmetric C-H stretching, C=H bending, C=O, C=C aromatic and C-O. Proton Nuclear Magnetic Resonance Spectrum (1H-NMR) of the isolate shows the presence of H-2' protons; H-5'; H-6'; H-6; H-8; OCH3/OH. From the results of the analysis and interpretation of the data, the isolated compounds obtained are flavonoid compounds of the flavonol group.