Isolasi dan Identifikai golongan senyawa fenolik dari bunga tumbuhan bungur besar(Lagerstroemia speciosa9L)Pers)

Abstract

The phenolic conipound had beeri isolated from the flo1;vers of the great crepe myrtle ( L. speciosa (L.) Pers ). The flowe,·s of the giant crepe were extracted by ,nace ration, with niethanol solvent, and the concentrated methanol extract was dissolved with ethyl acetate repeatedly until the solution was negative. Ethyl acetate concentrated extract was dissolved in methanol and partitioned witli n-liexane. The methanol extract was analyzed by th.in-layer chromatogra phy and separated by column chromatography using chlorofonn-ethyl acetate eliten,t 30:70, 20:80, 10:90 (vlv). Tlie compourid was obtain,ed as a greenish-yellow solid of 8,6 nig with ari Rf value of 0.62 with chloroform-ethyl acetate as eluent 30:70 (v/v ). From on the UV-Vis spectrum showing the presence maximwn absorption of a 111a velength (Amax) of 275 nm in bctnd I and 218 nm in band 11, the FT-JR spectrum shows the presence of OHJ C -H, C=O ester groitps, C=C aroniatic, C-Hsp3, C-0. Proton Nuclear Magnetic Resonance Spectrum (l H-NMR ) shows the presence of protons H-2 and H-6, protons fro,n CH3 bonded to the benzene ring, and protons of niethox y. Based on t/1,e interpretation of the data, the isolated compound is a phenolic compound belonging to the phenolic acid groitp, wliich is suspected to be methyl gallate