| dc.description.abstract | The use of doxorubicin (DOX) is effective as an antineoplastic in the
treatment of breast cancer but it has side effects especially cardiotoxic irreversible.
The cthyl acetate extract of leaves poguntano (EDP) and ethyl acetate extract of
andaliman fruit (EEABA) have anti breast cancer activity through inhibition of cell
cycle and apoptosis of the cells T47D and MCF-7 in vitro. The purposes of this
studies were to determine the anti breast cancer and cardioprotective activities of
EEADP and EEABA induced by doxorubicin in-vivo as well as antioxidant activity
assay of EEADP and EEABA.
The extracts were made by maceration graded using solvents based on the
level of polarity, starting from n-hexane and cthyl acetate and conducted the
phytochemical screening and then characterization of crude simplex and extracts. The
anticancer activity of EEADP and EEABA were tested to female mice. The doses
given were 125 mg/kg bw and 250 mg/kg bw. Animals induced with BaP 2 times a
weck for 4 weeks. Giving EEADP and EEABA were conducted from 1st week until
the last observation (11 week). Then IHC examination of Ki-67 and caspase-3 and
then scores of Ki-67 and caspase-3 expression were calculated. Cardioprotective
activity were tested to female rats induced doxorubicin HCI dosing of 20 mg/kg on 8
and 9th days. EEABA and EEADP with dose of 300 mg/kg were given from 19 day
until the 9th days. Measured levels of cTnT and CK-MB and cardiac histological
examination. Antioxidant activity test of EEABA EEADP were done by using DPPH
method. Concentration of each extract was 25 ppm, 50 ppm, 100 ppm, 200 ppm.
Uptake of DPPH solution was measured using a visible spectrophotometer.
The results of Ki-67 score expression by EEADP and EEABA with dose of
125 mg/kg bw were 35.180 and 28.505; whereas caspase-3 score expression were
14.791 and 35.611. The Ki-67 score expression by EEADP and EEABA with dose of
250 mg/ kg bw were 30.349 and 17.428, where as caspase-3 score expression were
33,88 and 85,574. The higher dose of each extracts, the more inhibits Ki-67
expression and the higher increases caspase-3 expression. EEABA is more active as
anticancer than EEADP. cTaT levels of EEADP + DOX and EEABA + DOX were
1.45 and 0.26 ; while CK-MB levels of EEADP + DOX and EEABA + DOX were
230 and 138. Histopathological analysis of rat's heart tissue of EEADP + DOX
showed bleeding, fragmentation, and less of miositolisis; while EEABA + DOX
group showed less of miositolisis and there were no some piknosis, bleeding, and
fragmentations. The higher dose of cach extracts, the lower ¢TnT and CK-MB levels
and the higher protective effect toward heart damage. EEABA is more active as
cardioprotective than EEADP. In antioxidant activity, IC50 of EEADP were 115.038
ppm and IC5o of EEABA were 135.376 ppm. | en_US |
