Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Tumbuhan Kareumbi (Homalanthus Populneus (Geiseler) Pax)

Abstract

Isolation and identification of flavonoid compounds from the stem bark of Kareumbi (Homalanthus populneus (Geiseler) Pax) through maceration extraction with methanol solvent has been carried out. The concentrated methanol extract was extracted again using ethyl acetate repeatedly until it was negative for 5% FeCl3. The ethyl acetate extract was concentrated and redissolved with methanol and then partitioned with n-hexane until clear. The methanol layer is then evaporated until all the methanol has evaporated. The concentrated methanol extract was then analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform : methanol (90:10, 80:20, 70:30, and 60:40) v/v as the mobile phase. The pure compound obtained was in the form of a yellowish-white amorphous solid, mass = 10 mg with an Rf value = 0.4 using chloroform: methanol 70:30 v/v as the eluent. Based on the UV-Visible spectrum, it shows a wavelength (λ max) of 330 nm in band I and 283 nm in band II. The FT-IR spectrum showed the presence of OH, C-H, C=O, C=C aromatic, C=H bending and C-O groups. The Proton Nuclear Magnetic Resonance Spectrum (1H-NMR) shows the presence of H-2' protons; H-6'; H-6 ; H-8 ; OCH3. Based on the data analysis and interpretation of the data, the isolated compound is a flavonoid compound in the flavonol group