Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Daun Tumbuhan Rumput Knop (Hyptis Capitata Jacq.)

Abstract

Flavonoid compounds have been isolated from the leaves of Knop grass (Hyptis capitata Jacq.). Isolation was carried out by maceration extraction method with methanol solvent and the methanol extract was extracted again with ethyl acetate repeatedly until it was negative for 5% FeCl3. The ethyl acetate extract was concentrated and redissolved with methanol then partitioned with n-hexane until the n-hexane layer was clear. The concentrated methanol extract was analyzed by thin layer chromatography and separated by column chromatography with chloroform : methanol (90:10; 80:20; 70:30; 60:40) v/v as eluent. Fraction 60-129 was purified by preparative thin layer chromatography using eluent chloroform: methanol (80:20) v/v to produce 10.3 mg of yellow amorphous solid with Rf 0.6 using eluent chloroform: methanol (80:20) v/v as Rf 0.6 using and chloroform eluent: ethylacetate (20:80) v/v. Based on the analysis of the UV-Visible Spectrophotometer showing a wavelength (λ max) of 332 nm, the FT-IR spectrum shows the presence of OH groups, C=C aromatic, C=O ketons, CH and CO. Proton Nuclear Magnetic Resonance Spectrum (1H-NMR) shows the presence of protons H-3, protons from CH3 bonded to the benzene ring, and protons of methoxy. Based on data analysis and data interpretation, it is suspected that the isolated compound is a flavonoid compound of the flavon group.