Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Tumbuhan Teh (Camellia sinensis (L) Kuntze)

Abstract

Isolation and identification of the class of flavonoid compound contained in the bark of the tea plant (Camellia sinensis (L) Kuntze) through stage by maceration extraction using methanol as a solvent. The concentrated methanol extract was re extracted using ethyl acetate repeatedly until 5% FeCl3 was negative. The ethyl acetate extract was concentrated and redissolved with methanol then partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was separated by column chromatography with silica gel as the stationary phase and ethyl acetate chloroform as the mobile phase (90:10; 80:20; 70:30; 60:40) v/v. The compound obtained from fraction 234-275 was purified by preparative thin layer chromatography to produce a yellow amorphous solid of 8.3 mg with an Rf value of 0.5 with chloroform as the eluent: ethyl acetate (10:90) v/v.Analysis of the compound obtained was carried out by spectrophotometry UV-Vis, FTIR, and 'H-NMR. The UV Vis spectrum shows a wavelength (max) of 280 in band II. The FT-IR spectrum shows the presence of -OH stretching groups.C-H stretching sp3. C-O Stretch, C-C Stretching, C-H Bending, C-O Stretch successively at wave numbers 3446.79 cm; 2922.16 cm; 1610.56 cm; 1512.19 cm; 1381.03 cm; 1251.80 cm . The ¹H-NMR spectrum shows the presence of H-2 protons; H-5; H-6; H-8; H-2'; H-5'; H-6'. Based on data analysis and interpretation of data, the isolated compound is a flavonoid compound of the isoflavone group.