Pengaruh Vitamin B6 terhadap Sitokin Proinflamasi TNF-Α pada Pergerakan Gigi Ortodonti pada Tikus Wistar

Abstract

Orthodontic treatment in correcting malocclusion involves an inflammatory process to move the teeth. Excessive and uncontrolled inflammatory processes have a negative impact on teeth and periodontal tissue. Anti-inflammatory agents can be used to reduce the ongoing inflammation. This study aims to determine the effect of vitamin B6 supplementation as an antiinflammatory in reducing proinflammatory cytokine TNF-α levels which does not reduce the quality of tooth movement so, it is hoped that vitamin B6 supplementation can be used as an alternative to anti-inflammatory medication.The research design was a laboratory experiment with a pretest and posttest design with a control group. Twenty-four Wistar rats were divided into 3 groups with 3 subgroups. The distance between Wistar rats' tooth movement was measured using digital calipers with split mouth method, while the method for analyzing the quantity of TNF-α proinflammatory cytokines was using ELISA. The mean value of tooth movement increased since day 1 in the control group, while the mean value in the treatment group was less structured. The results of the ANOVA test showed that there was a significant difference in orthodontic tooth movement, while there was no significant difference in TNF-α levels between the control group and the treatment group that was given vitamin B6.The study concluded that vitamin B6 supplementation reduced levels of the pro-inflammatory cytokine TNF-α thereby inhibiting orthodontic tooth movement.