Uji Daya Hambat Ekstrak Kulit Buah Delima (Punica Granatum) terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro

Abstract

Staphylococcus aureus is a bacterium that contributes to the development of oral abscesses. If this oral abscess is not treated right away, odontogenic infection will develop. Antimicrobial drugs can be used to prevent oral abscesses. Antimicrobial substances made from natural materials are increasingly being used because they can make chemical drugs less effective due to side effects and drug resistance. Pomegranates are among the natural elements that may help prevent oral abscesses. Pomegranate peels contains active compounds that can be utilized by the community as a medicine for healing abscesses. It is widely known that pomegranate peel extract contains tannins, saponins, and flavonoids. The study's purpose was to determine inhibitory ability of pomegranate peel extract on the growth of Staphylococcus aureus. This kind of study is a laboratory experiment with a post-test only control group design. The pomegranate peel extract was evaluated at concentrations of 3,125%, 6,25%, 12,5%, 25%, and 50% with DMSO as a control with four repititions. Kirby-Baurer Disc Diffusion is the technique used to test the antibacterial efficacy. The Kruskal Wallis test findings revealed a p value of 0.001 on inhibition, indicating that pomegranate peel extract had the ability to stop Staphylococcus aureus bacteria from growing. The average value of the inhibition zone that resulted was 9.03 mm at 3.125% concentration, 9.63 mm at 6.25% concentration, 10.98 mm at 12.5% concentration, 15.03 mm at 25% concentration, and 18.3 mm at 50% concentration. According to the study's findings, pomegranate peel extract can inhibit the growth of Staphylococcus aureus.