Pengaruh Beberapa Konsentrasi Ekstrak Kulit Biji Asam Jawa (Tamarindus i. Linn) Pada Candida albicans ATCC® 10231TM (In Vitro)

Abstract

Candida albicans is a normal flora can become a pathogen called opportunistic infection. Tamarind seed coat (Tamarind indica L.) has antifungal substances in the form of polyphenols, taninns, anthocyanin, dan oligomeric proanthocyanidin. The predisposing factors such as the use of dentures that are not removed and cleaned can cause denture stomatitis. The purpose of this study was to determine the zone of inhibition, Minimum Inhibitory Concentration (MIC), and Minimum Fungicidal Concentration (MFC) of tamarind seed coat extract concentration 3,125%, 6,12%, 12,5%, 25%, dan 50%, Chlorhexidine gluconate 0,2% as positive control, and DMSO as a negative control against Candida albicans ATCC® 10231TM. The experimental laboratory research method with a post test only control group design. The inhibition zone by the blank disk diffusion method was measured using digital caliper Kirby Bauer, while the MIC and MFC tests used the dilution method with Standard Mc Farland. Tamarind seed coat were extracted by maceration technique from each concentration added to the fungal suspension, repetition was carried out 4 times and then observations were made. Data analysis used the Kruskall wallis test followed by the Mann-Whitney. The results of the study obtained the value zone of inhibition of concentrations of 3.125%, 6.25%, 12.5%, 25%, and 50% on average 10,19 mm, 12,13 mm, 15,50 mm, 18,56 mm, dan 24,56 mm, and Minimum Inhibitory Concentration (MIC) 12,5%, Minimum Fungicidal Concentration (MFC) 25%. The conclusion is that there was the zone of inhibition, Minimum Fungicidal Concentration (MFC), and Minimum Inhibitory Concentration (MIC) the effect of tamarind seed coat extract on the growth of Candida albicans ATCC® 10231TM with a significance value (p=0,001).