Potensi Daya Hambat Bakteri Asam Laktat yang Diisolasi dari Terasi Udang Rebon (Acetes Indicus) dari Kecamatan Gebang Kabupaten Langkat Terhadap Bakteri Patogen

Abstract

Rebon shrimp paste is a fermented product with raw materials of rebon shrimp with salt which is generally done in Indonesia. The active compound produced by lactic acid bacteria is called bacteriocin. Bacteriocins are active compounds produced by bacteria including lactic acid bacteria that function as bactericides. Traditionally made rebon shrimp paste products have different bacteria and characteristics. The objectives of this study are as follows, to determine the type of lactic acid bacteria isolated from shrimp paste (Acetes indicus) produced from Gebang District, Langkat Regency. To determine the inhibitory power of LAB isolates isolated from shrimp paste (Acetes indicus) from Gebang District, Langkat Regency against pathogenic bacteria. Research procedures are sampling techniques, sample preparation techniques, isolation of lactic acid bacteria, morphological characterization of lactic acid bacteria, gram staining, observation of LAB morphological characters, physiological characterization of LAB, catalase test, triple sugar iron agar (TSIA) test, Motility test, identification of Lactic Acid Bacteria, Antagonistic Inhibitory Test of LAB Isolates against Pathogenic Bacteria Isolation of LAB from 3 processing shrimp paste located in Gebang District, Langkat Regency with the aim of growing LAB colonies on MRSA media with the addition of 1% CaCO3. Lactic acid bacteria isolation was carried out using the pour cup method for 3 repetitions and then incubated at 37oC for 2 days. Based on the observation of LAB isolation grown on MRSA media presented. The results of bacteria from the isolation of shrimp paste rebon from Gebang District, Langkat Regency, types of lactic acid bacteria with the genus are Bacillus sp., Streptococcus sp., Lactobacillus sp., Brevibacterium sp., Micrococcus sp. and Corynebacterium sp. The results of the inhibition of LAB isolates from shrimp paste rebon (Acetes indicus) from Gebang District, Langkat Regency against Eschericia coli and salmonella in the Terasi Processing Unit are all three categorized as strong