Pengaruh Penambahan Gel Essential Oil Oregano (Origanum vulgare) dengan Konsentrasi dan Waktu Pemakaian yang Berbeda terhadap Kekasaran Permukaan Soft Denture Liner Silikon Swapolimerisasi

Abstract

Soft denture liner is a material that forms a cushioned layer between the hard denture base on the oral mucosa. But, soft denture liner accomponied by several problems such as the failure bonding between soft denture liner and the denture base, and the increased sur face roughness causing porosity. Changes in surfaace roughness cause Candida colonization that compromise the material’s durability. The method that has been used to prevent Candida is the mechanical method, but this method is not recommended for soft denture liners because it can cause an increase in surface roughness. An alternative method to prevent Candida colonization was incorporating natural anti fungal agent, namely Origanum vulgare essential oil gel with concentration 3,12% and 6,25%. This study aims to determine the effect of addition Origanum vulgare essential oil gel with different concentrations and usage duration on surface roughness of auto-polymerized silicone soft denture liner. The sample used in this study was auto-polymerized silicone soft denture liner which was made using a cylindrical metal mold with a diameter of 15 mm and thickness of 10 mm. A total of 40 samples (N=40) were divided into 8 groups (n=5). Samples were placed in an incubator at 37℃ for 7 days and 14 days as a simulation of oral conditions. Measurement of surface roughness was tested using Profilometer. Data were analyzed using the univariate test to determine the mean value and standard deviation of surface roughness for each group. One-way Anova to determine the effect of addition Origanum vulgare essential oil gel with concentrations 3,12% and 6,25% based on usage duration of 7 days on surface roughness of auto-polymerized silicone soft denture liner. One-way Anova and Dunnett’s T3 to determine the effect of addition Origanum vulgare essential oil gel with concentrations 3,12% and 6,25% based on usage duration of 14 days on surface roughness of auto-polymerized silicone soft denture liner. The result from univariant test was obtained the mean and standard deviation based on 7- day usage duration in negative control group was 0,16 ± 0,09 μm, the nystatin-added group was 0,13 ± 0,11 μm, the 3,12% oregano essential oil gel-added group was 0,06 ± 0,03 μm and the 6,25% oregano essential oil gel-added group was 0,06 ± 0,007 μm. The mean and standard deviation based on 14-day usage duration in negative control group was 0,33 ± 0,07 μm, the nystatin-added group was 0,69 ± 0,21 μm, the 3,12% oregano essential oil gel-added group was 0,12 ± 0,02 μm and the 6,25% oregano essential oil gel-added group was 0,08 ± 0,01 μm. Based on one-way Anova analysis showed there was no effect in the addition of nystatin and Origanum vulgare essential oil gel with concentration 3,12% and 6,25% based on usage duration of 7 days on surface roughness of auto-polymerized silicone soft denture liner with pvalue= 0,080(p>0,05). Based on one-way Anova analysis showed there was an effect in the addition of nystatin and Origanum vulgare essential oil gel with concentration 3,12% and 6,25% based on usage duration of 14 days on surface roughness of autopolymerized silicone soft denture liner with p-value=0,001 (p<0,05). From the results on this study it can be concluded that the addition of Origanum vulgare essential oil gel with concentration 6.25% for 14 days it is recommended to use auto-polymerized silicone soft denture liners because it can reduce surface roughness