| dc.description.abstract | The aims of this study were to obtain the best ratio of chitosan and catechins in the
development of bioplastic films based on PLA-PCL related to the characteristics,
physical properties, mechanical properties, activity of Staphylococcus aureus and
Escherichia coli bacteria. These characteristics are related to the application of beef
and pineapple to Total Volatile Basic Nitrogen (TVB-N) and vitamin C. The
production of bioplastics was carried out by extrusion and thermo-compression
methods at 160 0C. Chitosan as an antibacterial agent is purchased commercially,
while catechins as an antioxidant agent are obtained through the jamblang seed
extraction process. Extraction was carried out using socket at 55 0C for 6 hours. The
antioxidant content of jamblang seed extract was confirmed using GC-MS. The
characterization results showed that there were 8 chemical components belongs to
catechins with the main components being β-humulen, α-guaiene and
methandrostenolone. The antioxidant activity of 53% of jamblang seed extract was
determined by the DPPH (2,2′-diphenyl-1-picrylhydrazyl) method. The results of
the analysis of the bioplastic functional groups showed a shift in the absorption
bands of the functional groups (C-O), (C-H) and (C=C) indicating interactions
between the components of the PLA-PCL-PEG chitosan/catechin bioplastics. The
interaction between PLA-PCL-PEG and chitosan produces a peak of the amine
group (N-H). Stretching of the carbonyl group (C=O) has also occurred in all
samples containing a mixture of catechins and chitosan. The highest tensile strength
value was obtained from the bioplastic contains 0.5 g chitosan/0.5 mL catechin
(44.69 MPa) according to the surface shown by Scanning Electron Microscopy
(SEM) with very small voids. The best WVTR value also shown by the bioplastic
contains 0.5 g chitosan/0.5 mL catechin sample of 1.38 g/m2
/hour. The highest
reduction of gram-positive bacteria S. aureus (96.66%) and gram-negative bacteria
E. coli (73.98%) resulted from samples of bioplastic contain 0.6 g chitosan/0.4 mL
catechins. The application of bioplastic contains 0.6 g chitosan/0.4 mL catechins
was able to slow down the increase in TVB-N in beef and slow down the decrease
in vitamin C content in pineapple during 15 days of storage | en_US |
