Penyediaan Pembalut Luka Diabetes Berbasis Selulosa Bakteri dengan Impregnasi Nanoenkapsulasi Andaliman (Zanthoxylum acanthopodium) Kurkumin (Curcuma longa) Sebagai Antiinflamasi dan Antibakteri

Abstract

Diabetes mellitus is a chronic disease and usually causes wounds caused by infection due to high blood glucose levels, making it a strategic place for the growth of germs. Wounds in diabetics can be treated early to prevent further complications by using wound dressings that can control fluid from the wound and have antibacterial properties. Bacterial cellulose was modified by carrying out an impregnation process with the addition of nanoencapsulated andaliman and curcumin to increase the ability as antibacterial and anti-inflammatory. The SB impregnation process was carried out by varying the ratio of andaliman curcumin with a ratio of 1:0, 1:1, 1:2, 1:3 and 1:4. This research is focused on making andaliman nano-encapsulated wound dressings for diabetes mellitus wounds with curcumin variations in nano-encapsulation. The results of the Pasrticle Size Analyzer test showed that the size of the Andaliman curcumin nanoencapsulation was 0.03603 μm and an average of 0.02159 μm, which is good for drug delivery processes. The results of SEM and FTIR analysis showed that the results of SB impregnation with the addition of andaliman curcumin nanoencapsulation went well. Based on the results of FTIR analysis obtained 3 absorbance bands 1690 cm-1, 2914 cm-1 and 3347 cm-1. This functional group is also read on the spectrum at a ratio of SB 1:0 to SB 1:4. The appearance of the image in the SEM analysis shows that the surface of the SB fiber looks uneven and the clumps bound to the SB pores are increasing at SB 1:1 to 1:4. Based on the results of XRD analysis with the addition of andaliman curcumin nanoencapsulation to SB, it showed successful ex-situ impregnation, namely the presence of 2 peaks at 11° and 22°. The results of the antibacterial test at SB 1:4 showed the highest results with an inhibition zone diameter of 12.3 mm in Staphylococcus aureus and 7 mm in Escherichia coli. The water content and porosity of the andaliman-curcumin nanoencapsulated SB decreased but were still able to fulfill its use as a wound dressing. Another aspect that supports it can be used as a wound dressing material, namely the results of testing for diabetic wound closure at SB 1:4 showed a maximum result of 78.89% on the 17th day.