Analisis Secara Simultan Campuran Vitamin C dan Zink Sulfat Dalam Sediaan Tablet Secara Spectrofotometri Visible Dengan Metode Dual Wavelength dan Ratio Subtraction

Abstract

Analysis is a study starting from the separation, identification, and quantification of chemical components in natural and artificial materials. Analysis is usually used to examine compounds in a mixture of drugs and compounds in natural products. Both Vitamin C and Zinc Sulfate are combined in a drug preparation because Zink Sulfate helps the absorption of Vitamin C. Most of the drugs in circulation are combinations of several active ingredients, each of which aims to enhance the therapeutic effect of the drug and ease of use. Determination of the levels of efficacious substances in drug preparations is an important part, and for simultaneous examination of a mixture of Vitamin C and Zinc Sulfate in tablet preparations by Visible spectrophotometry has never been done.. This study aims to develop the Visible Spectrophotometry method using the multiple wavelength method and the ratio reduction method in the simultaneous analysis of Vitamin C and Zinc on tablet preparations without separation. The dual wavelength method is the use of two selected wavelengths for each drug in such a way that the difference in absorbance is zero (∆A=A2-A1) for one drug. While the ratio subtraction method is a method applied to the analysis of a mixture of two drugs X and Y having overlapping spectra and one of them is extended, to be able to determine X by dividing the spectrum of the mixture by the concentration of Y which is known as a divider (Y0). The research was carried out by optimizing the type of solvent, namely a mixture of Methanol: Water 50: 50 with the addition of 0.01% addition complexing agent and Methanol phosphate buffer pH 4; pH 5; pH 6 with the addition of 0.01% addition complexing agent. The spectrophotometric method The double technique visible wavelength method and the ratio reduction method were then tested for validity based on parameter validation, namely linearity, accuracy, precision, LOD and LOQ as well as intraday and interday. Then, this method was applied to determine the levels of Vitamin C and Zinc in tablet preparations. The results showed that a mixture of methanol: water with a ratio of 50:50 with the addition of 0.01% addition complexing agent was the best solvent used as a solvent for analysis. Application of the dual-wavelength method for assays was carried out at λ 477 nm and 500 nm for Vitamin C and Zinc, with yields of 100.0% and 101.0% for Vitamin C and Zinc. In the ratio application method, the levels of Vitamin C and Zinc were determined at λ 477 nm and 500 nm respectively, with yields of 102.50% and 104.31% for Vitamin C and Zinc. Qualified parameter validation. The conclusion is that the multiple wavelength method and the ratio reduction method can be used to determine simultaneous levels of Vitamin C and Zinc using a mixture of Methanol: water at a ratio of 50: 50 with the addition of a complexing agent 0.01% and the results meet the requirements of FI ed VI and meet the validation of the parameter requirements, namely linearity, accuracy, precision, LOD and LOQ as well as intraday and interday.