| dc.description.abstract | Analysis is a study starting from the separation, identification, and quantification
of chemical components in natural and artificial materials. Analysis is usually used to
examine compounds in a mixture of drugs and compounds in natural products. Both
Vitamin C and Zinc Sulfate are combined in a drug preparation because Zink Sulfate
helps the absorption of Vitamin C. Most of the drugs in circulation are combinations of several active ingredients,
each of which aims to enhance the therapeutic effect of the drug and ease of use.
Determination of the levels of efficacious substances in drug preparations is an
important part, and for simultaneous examination of a mixture of Vitamin C and Zinc
Sulfate in tablet preparations by Visible spectrophotometry has never been done..
This study aims to develop the Visible Spectrophotometry method using the
multiple wavelength method and the ratio reduction method in the simultaneous analysis
of Vitamin C and Zinc on tablet preparations without separation. The dual wavelength
method is the use of two selected wavelengths for each drug in such a way that the
difference in absorbance is zero (∆A=A2-A1) for one drug. While the ratio subtraction
method is a method applied to the analysis of a mixture of two drugs X and Y having
overlapping spectra and one of them is extended, to be able to determine X by dividing
the spectrum of the mixture by the concentration of Y which is known as a divider (Y0).
The research was carried out by optimizing the type of solvent, namely a mixture of
Methanol: Water 50: 50 with the addition of 0.01% addition complexing agent and
Methanol phosphate buffer pH 4; pH 5; pH 6 with the addition of 0.01% addition
complexing agent. The spectrophotometric method The double technique visible
wavelength method and the ratio reduction method were then tested for validity based
on parameter validation, namely linearity, accuracy, precision, LOD and LOQ as well
as intraday and interday. Then, this method was applied to determine the levels of
Vitamin C and Zinc in tablet preparations.
The results showed that a mixture of methanol: water with a ratio of 50:50 with the
addition of 0.01% addition complexing agent was the best solvent used as a solvent for
analysis. Application of the dual-wavelength method for assays was carried out at λ 477
nm and 500 nm for Vitamin C and Zinc, with yields of 100.0% and 101.0% for Vitamin
C and Zinc. In the ratio application method, the levels of Vitamin C and Zinc were
determined at λ 477 nm and 500 nm respectively, with yields of 102.50% and 104.31%
for Vitamin C and Zinc. Qualified parameter validation.
The conclusion is that the multiple wavelength method and the ratio reduction
method can be used to determine simultaneous levels of Vitamin C and Zinc using a
mixture of Methanol: water at a ratio of 50: 50 with the addition of a complexing agent
0.01% and the results meet the requirements of FI ed VI and meet the validation of the
parameter requirements, namely linearity, accuracy, precision, LOD and LOQ as well
as intraday and interday. | en_US |
