Identifikasi Genetik Ikan Pari Kupu-Kupu (Gymnura sp.) Berdasarkan DNA Barcoding yang Didaratkan di Tempat Pendaratan Ikan (TPI) Pantai Labu Kabupaten Deli Serdang

Abstract

This research in respect of the analysis of DNA barcoding in butterfly stingrays (Gymnura sp.) which was landed in TPI Pantai Labu, Deli Serdang Regency. The purpose of this research is to find out molecular genetic identification and phylogenetic analysis of butterfly stingrays landed in TPI, Deli Serdang Regency. This study used the DNA barcoding method by taking samples and sample analysis was carried out at the Laboratory of the Indonesian Biology Generation Foundation, Biology and Molecular Division, East Java. The results of molecular identification in sample 1 and sample 2 are species of Gymnura poecilura with Total Score 669, Query cover 100%, E-Value 0.0, per. Ident 92.44% and sample 2 with Total Score 860, Query cover 100%, E-Value 0.0, per. Identities 99.78%. The fish sample studied has a close kinship with the resulting outgroup which has the closest genetic distance of 0.0793 where the calculation of genetic distance is by determining proximity with a numeric number 0-1, with the provision that the closer to 0 the closer the genetic distance.