Pengaruh Perendaman Basis Gigi Tiruan Nilon Termoplastik dan Polyetheretherketone dalam Larutan Alkalin Peroksida dan Castor Oil terhadap Jumlah Candida Albicans dan Kekasaran Permukaan

Abstract

Thermoplastic nylon has several problems such as water absorption, surface roughness, bacterial contamination, discoloration and is more difficult to polish because of its low melting point. Polyetheretherketone (PEEK) with addition of ceramic particles is intended to increase the ability to be polished properly. Many plants that have been known and studied have antimicrobial effects and have good biocompatibility and can be obtained at relatively low prices. One example is castor oil. Castor oil has good characteristics as a denture cleanser because it has good detergent properties and anti-microbial activity that can break down glucose molecules from the cell walls of pathogenic microbes and fungi as well as tastes and odors that can be tolerated by patients. The denture cleaning agent that is commonly used is alkaline peroxide in tablet preparations. Alkaline peroxide is effective in dissolving plaque due to its bactericidal and fungicidal properties. However, the use of alkaline peroxide cleaning agents was reported to cause changes in the morphology of the denture base surface due to the release of active oxygen. This study aims to see the effect of immersing thermoplastic nylon and polyetheretherketone denture bases in alkaline peroxide solution and castor oil on the amount of Candida albicans and surface roughness. The research materials used are thermoplastic nylon and polyetheretherketone. The types of cleaning agents used were alkaline peroxide and 10% castor oil. Samples for measuring the number of Candida albicans and surface roughness totaled 30 pieces with a diameter of 20 mm and a thickness of 2 mm. The samples were then divided into 6 groups (n=5), namely each group of thermoplastic nylon and polyetheretherketone which were soaked in each cleaning solution of alkaline peroxide and 10% castor oil. Calculation of the Candida albicans count was carried out using a colony counter in CFU/mL, while for measuring surface roughness a profilometer was used on the same surface area before and after immersion in μm units. The results of the Candida albicans count, in the thermoplastic nylon group which were soaked in a solution of alkaline peroxide and 10% castor oil, showed a p value = 0.0001 which indicated the effect of immersing the nylon material in the cleaning solution. Likewise, by immersing the PEEK material in an alkaline peroxide and 10% castor oil, it showed a p value = 0.0001. The Candida albicans count in the alkaline peroxide group was lower than that of 10% castor oil, but the Candida albicans count in 10% castor oil showed a significant decrease compared to distilled water. This is caused by differences in the pH of cleaning solutions that affect the amount of Candida albicans. The results of the surface roughness test, in the thermoplastic nylon group which were soaked in a solution of alkaline peroxide and 10% castor oil, showed a value of p = 0.0001 which indicated the effect of immersing the nylon material in the cleaning solution. Likewise, by immersing the PEEK material in an alkaline peroxide and 10% castor oil, it showed a p value = 0.001. The results of the paired t-test of PEEK in 10% castor oil before and after immersion showed a value of p = 0.061 (p> 0.05, which means there was no effect of PEEK immersion in 10% castor oil). This is because oil-based cleaning agents will reduce changes in material properties. From the results of this study, it can be concluded that the PEEK denture base soaked in 10% castor oil solution showed the least change in surface roughness and was able to effectively eliminate Candida albicans.